Study done to analyze germ population on the hands of ICU HCWs and patients, also to analyze alteration in germs population of ICU HCWs hands when arrived at hospital, after patient contact and one hour after hand hygiene with alcohol-based formulation
Research done with two stages with cross-sectional study, and continued with pre & post test study design using quantitative approach. Sampling was conducted with 18 ICU HCWs hands and ICU patients. For HCW, sample was collected as mentioned above. For patient, specimens were collected from blood, urine, pus, sputum in study period.
Distribution of germs population on the hands of HCWs are gram-negative 93.54%(n=29), gram-positive 6.45%(n=2), distribution of germs population in ICU patients are gram-negative 78.26%(n=36) and gram positive 21.73%(n=10). WHONET analysis showed the germs population when HCWs arrived was gram negative 92%(n=12) and antibiotic-resistant gram positive 8%(n=1), the species are Acinetobacter, Klebsiella, Pseudomonas, Enterobacter, Staphylococcus. After patient contact was found antibiotic-resistant gram negative 100%(n=17), the species are Enterobacter, Acinetobacter, Pseudomonas, Klebsiella. Moreover, one hour after hand hygiene with alcohol-based formulation was found gram negative 90%(n=10) and antibiotic-resistant gram positive 9%(n=1), the species are Enterobacter, Pseudomonas, Klebsiella, Acinetobacter, Staphylococcus.
Distribution of germs population on the hands of HCW and ICU’s patients was dominated by gram negative with antibiotic resistant. There was alteration in germs population on hands when HCW arrived at hospital, after patient contact and one hour after hand hygiene. Suggest to do hand hygiene immediately before and after contact with patient and patient's surrounding.